METHOD FOR MEASURING IMMUNE REACTION BY USING FLUCTUATION OF LIGHT INTENSITY

摘要

PURPOSE:To improve S/N and measurement accuracy by converting pho toelectrically the scattered light from a cell, taking out the output synchronized with the chopped light out of the outputs thereof and determining the power spectral density of the intensity fluctuation. CONSTITUTION:Antigen-antibody reaction arises in a cell 7 and the light scat tered by the pulverous particles formed by such reaction is made incident through a collimator 10 having a pair of pincholes to a photodetector 11 consisting of a photoelectron multiplier. The output monitor light of a photode tector 8 is supplied to a data processing unit 14 through a lock-in amplifier 103 which takes out only the light passed actually through a reference cell 102 in synchronization with the chopping operation of a chopper 101. The output signal from the photodetector 11 is supplied to the unit 14 through a lock-in amplifier 104 which takes out only the light passed actually through the cell 7 in sychronization with the chopping operation of the chopper 101. The result of the antigen-antibody reaction is outputted by the unit 14.