发明名称 |
Method of cloning modified streptomycetes DNA. |
摘要 |
<p>A method of cloning endogenously modified Streptomycetes DNA, which is normally rejected by restrictionless heterospecific hosts, is disclosed. The method uses bacteriophage lambda to construct a genomic library of modified Streptomycetes DNA; such lambda-containing Streptomycetes DNA is replicated to provide a source of non-modified Streptomycetes DNA. This non-modified DNA is subcloned into a selectable cloning vector and used to transform restrictionless heterospecific hosts. The transformants can then be screened for clones containing genes of interest.</p> |
申请公布号 |
EP0176340(A1) |
申请公布日期 |
1986.04.02 |
申请号 |
EP19850306763 |
申请日期 |
1985.09.24 |
申请人 |
ELI LILLY AND COMPANY |
发明人 |
HERSHBERGER, CHARLES LEE;LARSON, JEFFREY LYNN |
分类号 |
C12N15/09;C12N1/20;C12N15/63;C12N15/76;C12R1/19;C12R1/465;(IPC1-7):C12N15/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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