Apparatus and method for optimizing separation of an unknown sample by liquid chromatography

摘要

An apparatus and method are disclosed to optimize resolution of unknown samples by liquid chromatography. A plurality of resolution experiments are performed where the solvents in the liquid chromatograph column are changed from one experiment to the next. The chromatogram for each experiment is analyzed to measure its peak resolution. A measure of peak resolution is displayed for each chromatograph thereby allowing the operator to select easily the chromatograms with the required peak resolution and determine the solvent conditions required to produce that resolution.