NEW P-PHENYLENEDIAMINE PEPTIDES AND REACTANCE CONTAINING THEM FOR THE DETERMINATION OF PROTEASES OF THE BLOOD COAGULATION SYSTEM

摘要

New compositions having the general formula (I), wherein A is the amino acid arginine or lysine, X is an N-terminal amino acid protection group or D-amino acid, Y is a single bound or a chain comprised of 1-3 amino acids, NR1R2 is a group in the o-position or p-position wherein R1 and R2 are independent from each other and represent hydrogen or alkyl having from 1 to 3 atoms of carbon and R3 is a hydrogen atom, a carboxyl group, a halogen atom or an alkyl group having from 1 to 3 atoms of carbon. Said compositions are useful as chromogenic substrates of proteases. They are obtained by reduction and optionally by alkylation of corresponding nitroanilides. A corresponding reactant to determine proteases of the blood coagulation system comprises a composition having the formula (I), an oxidation agent, an anyline-or phenol derivative which forms a dye stuff by oxidation with the phenylene diamine and a buffer substance.