| 摘要 |
There is claimed, as a new composition of matter, recombinant DNA containing an amylase coding gene, prepared by the in vitro process of cleaving DNA derived from a bacterial donor microorganism and combining the resulting DNA fragments with a vector, which has been similarly cleaved, characterized in that said vector comprises a plasmid or the DNA of a derivative of phage lambda. Also claimed are genetically engineered microorganisms containing the novel DNA, processes for preparing the novel DNA, and novel amylase enzymes, their production from the DNA and their use in hydrolysing starch, …<??>The donor microorganism is preferably a Bacillus or a Klebsiella strain. The vector may be a phage or a plasmid. The host microorganisrn is preferably E.coli or B. subtilis. The amylase enzyme produced is preferably an alpha-amylase, a beta-amylase or a pullulanase. |
