摘要 |
PCT No. PCT/SE85/00358 Sec. 371 Date May 16, 1986 Sec. 102(e) Date May 16, 1986 PCT Filed Sep. 18, 1985 PCT Pub. No. WO86/01843 PCT Pub. Date Mar. 27, 1986.A method is described of obtaining cellulase deficient strains which have great ability to degrade lignin at the same time. The method comprises crossing a homokaryotic cellulase deficient mutant of a white-rot fungus having a full sexual cycle with a homokaryotic strain of the same fungus, this strain having great lignin degrading ability. Strains are subsequently selected that have great lignin degrading ability in combination with cellulase deficiency. A suitable white-rot fungis is Phanerochaete chrysosporium. The crossings are suitably performed for a plurality of generations. The strain of white-rot fungus obtained is used for delignifying lignocellulosic material. |