GEL FOR AFFINITY CHROMATOGRAPHY HAVING GROUP SPECIFICITY AND ITS PRODUCTION

摘要

PURPOSE:To obtain a gel for chromatography which maintains a solid granular state, is insoluble in an aq. solvent and has excellent physical stability by esterifying non-crosslinked cellulose by sulfuric acid. CONSTITUTION:A esterifying agent such as sulfuric acid is dissolved in amine or amide and the granular material of the non-crosslinked cellulose is brought into reaction therewith and is then neutralized with an alkali. Sulfuric anhydride or chlorosulfonic acid is preferable as the esterifying agent such as sulfuric acid and is used at about 1-50pts. (pts.wt., hereafter the same) in 100pts. the raw material cellulose and the reaction is effected at about -10-100 deg.C for about 30min-6hr. The amine or amide to be used as the solvent is exemplified by pyridine, dimethylformamide, etc. and the raw material cellulose to be added thereto so as to react therewith is used after the commercially marketed granular material having about 15-150mum grain size for chromatography is dried to about 1% moisture content. The resultant gel for affinity chromatography consists of the water-insoluble solid granular particles of the non-crosslinked cellulose sulfate having 15-150mum grain size.