Extraction of anthocyanine(s) and flavonol(s) from vegetable material

摘要

The process consists of (a) lyophilising the vegetable material; (b) extracting with a methanol/HCl mixt. by ultrasonic action in presence of sodium metabisulphite; drying the crude extract under vacuum, taking up in TBA and injecting onto a chromatographic column of silica cellulose; (c) successive elution in TBA of a first fraction and in 15% acetic acid of the second fraction; (d) concentrating both fractions to dryness and dissolving the first in methanol and the second in ethanol/HCl mixt.; and (e) concn. of the two solns. to obtain flavanols and anthocyanins respectively.