| 摘要 |
PURPOSE:To decrease noise to measuring objects of a wide range and to obtain a spectral distribution of fluorescence with high accuracy by measuring repeatedly the spectral distribution of slight fluorescence in a single flow part. CONSTITUTION:The fluorescence emitted from the cells excited by an irradiating beam Lb which is controlled in the width of the X-direction and Z-direction by a circular columnar concave lens 10 and circular columnar convex lenses 11, 12 is condensed by an optical fiber group 13. When the cells labeled by the fluorescence enter the inside of the rectangular irradiating beam Lb, the intensity of the forward scattered light and the intensity of the backward scattered light are first measured by a means for measuring the scattered light and are taken through an interface 20 into a microprocessor unit MPU21. On the other hand, a one-dimensional photosensor array 17 is repeatedly scanned and the fluorescence spectral distribution is added 19 until the cells leave the beam Lb. The fluorescence spectral data of high S/N is taken through the interface 20 into the MPU 21. The repeatedly obtd. data on the scattered light and the data on the spectral distribution of the fluorescence are analyzed in the MPU21 and the result thereof is displayed 22. |
