| 摘要 |
The present invention relates to the regeneration of corn. The process comprises the steps of: (a) culturing tissue obtained from a corn plant on a first medium which comprises mineral salts, vitamins sucrose and a hormone in an amount sufficient to ensure callus formation; (b) subculturing the calli on a second medium which comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure callus maintenance; (c) subculturing the calli on a third medium which comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure shoot and root formation; and (d) optionally subculturing said shoots on a fourth medium which comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficent to ensure plantlet maturation including additional root formation, whereby plants are obtained. |
