| 摘要 |
<p>PURPOSE:To purify Japanese B encephalitis virus in high yield in high purity, by bringing a Japanese B encephalitis virus-containing solution into contact with cellulose sulfate gel, adsorbing the Japanese B encephalitis gel on the gel, eluting it from the gel. CONSTITUTION:In purity Japanese B encephalitis virus, a Japanese B encephalitis virus-containing solution (including virus obtained from brain material of animal infected with Japanese B encephalitis or culture material of infectious tissue culture cell, or Japanese B encephalitis virus material obtained by using gene recombinant) is brought into contact with cellulose sulfate gel, the Japanese B encephalitis virus is adsorbed on it, the Japanese B encephalitis virus is eluted from the cellulose sulfate gel, to purify the Japanese B encephalitis virus. Cellulose sulfate obtained from crystalline cellulose consisting of crystal region and amorphous region is preferable as the cellulose sulfate.</p> |
