发明名称 |
Plasmids containing lambda Pl promoter, and engineered restriction site for convenient replacement of ribosomal binding site, hosts containing the plasmids and related methods. |
摘要 |
(1) Vector that on introduction into a suitable bacterial host cell contg. the thermolabile repressor C (I) renders the host cell capable, when the temp. is raised to a level at which the repressor is inactivated, of effecting expression of a desired gene inserted in the vector to produce polypeptide (I) encoded by the gene is new when it comprises a double-stranded DNA molecule including in 5'- to 3'- order. (a) DNA sequence contg. the promoter and operator P(L)O(L) from lambda bacteriophage; (b) a N utilisation site for binding antiterminator N protein; (c) a first restriction enzyme site permitting replacement of the DNA sequence contg. the ribosomal binding site following after; (d) DNA sequence contg. a ribosomal binding site for making the MRNA of the desired gene capable of binding to ribosomes in the host cell; (e) ATG initiator codon or a DNA sequence converted into the codon on insertion of the desired gene into the vector; and (f) a second restriction enzyme site for inserting the desired gene into the vector in phase with the ATG initiation codon; and (g) a DNA sequence contg. an origin of replication from a bacterial plasmid capable of autonomous replication in the host cell; and for selection, DNA sequence contg. a gene associated with a selectable or identifiable phenotypic trait manifested when the vector is present in the host cell, or DNA sequence contg. fragment C (I) 434, including the gene for the C (I) 434 repressor protein and its associated promoter and operator sequence. |
申请公布号 |
EP0173280(A1) |
申请公布日期 |
1986.03.05 |
申请号 |
EP19850110683 |
申请日期 |
1985.08.26 |
申请人 |
BIO-TECHNOLOGY GENERAL CORPORATION |
发明人 |
AVIV, HAIM;BARTFELD, DANIEL;GORECKI, MARIAN;HARTMAN, JACOB R.;KANNER, DOV;LEVANON, AVIGDOR;LOCKER-GILADI, HILLA;OPPENHEIM, AMOS B. |
分类号 |
C12N15/09;A23K1/165;A61K35/74;A61K38/00;A61K38/27;A61K38/44;A61K38/45;C07H21/04;C07K14/005;C07K14/195;C07K14/575;C07K14/61;C07K14/775;C07K19/00;C12N1/20;C12N1/21;C12N9/02;C12N15/00;C12N15/02;C12N15/53;C12N15/69;C12N15/73;C12P21/02;C12P21/08;C12R1/19 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|