SPANGLE FOR PRESERVING MAMMAL EMBRYOS BY FREEZING AND APPLICATION THEREOF IN EMBRYONIC TRANSPLANTATION

摘要

The spangle (1) which is filled at both its ends (13, 14) until it is used in the animal contains an embryo (4') in a cryoprotector (4) which is surrounded respectively (A) on one hand, successively in the direction of one end (13) (a) with a gas mixture (5) (b) with a growth medium (6) and (c) with a first filling and plug (2) and (B) on the other hand, successively in the direction of the other end (14) (d) with a gas bubble (7), (e) with a sucrose solution, (f) with another gas bubble (9) and (g) with a second plug (3). Application to embryonic transplantation.