| 摘要 |
Antibodies which are specific in their binding properties toward mammalian ras gene products are described. The antibodies are obtained from p20 polypeptide extracted from chromatin characterized by a molecular weight in the range of 16,000 to 21,000 daltons and the following amino acid composition: aspartic+glutamic acids in the range of 21.8-25.5 mole %; lysine+arginine in the range of 13.5-21.4 mole % and a ratio of lysine to arginine in the range of 2.31-3.1. The p20 polypeptide specifically reacts with the polyclonal anti-p20 antibodies. The antibodies are immunoreactive with yeast p20 polypeptide and with native p21 ras related proteins expressed in tumor cells. Accordingly, the antibodies are suggested to serve as additional probes for assessing the expression of ras gene related proteins in human malignancy. |
