发明名称 |
COMPOSITION OF A NOVEL IMMUNOGEN FOR PROTECTION AGAINST DIARRHEAL DISEASE CAUSED BY ENTEROTOXIGENIC ESCHERICHIA COLI |
摘要 |
Novel immunogen which can be used for immunological protection against acute diarrheal disease caused by enterotoxigenic strains of Escherichia coli. The novel immunogen is provided by cross-linking the E. coli heat-stable enterotoxin with the E. coli heat-labile enterotoxin (either the complete holotoxin or just the B subunit of this toxin) by a conjugation process in the presence of a conjugating agent, typically the water soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide. This results in a unique new molecule in that the toxic properties of each individual toxin are greatly reduced, the heat-labile toxin (or its B subunit) retains its antigenicity, and the heat-stable toxin acquires immunogenicity as a funtion of the reaction. Immunization with the cross-linked immunogen provides immunological protection in mammals against water secretion induced by strains of E. coli which produce the heat-labile or heat-stable enterotoxins, either singly or together. |
申请公布号 |
EP0081582(B1) |
申请公布日期 |
1986.02.12 |
申请号 |
EP19820902387 |
申请日期 |
1982.06.03 |
申请人 |
THE UNIVERSITY OF ROCHESTER |
发明人 |
KLIPSTEIN, FREDERICK A.;ENGERT, RICHARD F.;CLEMENTS, JOHN D. |
分类号 |
A61K39/108;A61K;A61K39/106;A61K39/116 |
主分类号 |
A61K39/108 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|