Monoclonal antibody, process for preparing same, reagent for detecting cancer antigen containing the monoclonal antibody and process for preparing same.

摘要

A monoclonal antibody is described, having a specific reactivity with a cancer-associated antigen selected from pancreas cancer-associated antigen, intestinum crassum cancer-associated antigen and hepatoma-associated antigen and has the following characteristics:
<UnorderedLists id="ula01" listStyle="none"><ListItem>(1) it exists in a blood serum of pancreas cancer, intestinum crassum cancer and hepatoma patients;</ListItem><ListItem>(2) it comprises glycoproteins;</ListItem><ListItem>(3) it has a molecular weight of about 700 000 to 1 500 000, as measured by gel-filtration and determined by comparison with that of a known protein;</ListItem><ListItem>(4) it has a maximum absorption of 280 nm when dissolved in 0.1 M acetic acid buffer solution (pH 4.5); and</ListItem><ListItem>(5) it is released at least in a cultured supernatant medium of established cell line of COLO-201, TE-1, TE-2, TE-3, NRC-12, MKN-45 and KATO III. The monoclonal antibody is utilized as a reagent for detecting pancreas cancer, intestinum crassum cancer and hepatoma. A process for preparing the monoclonal antibody and a process for preparing the reagent are also disclosed.</ListItem></UnorderedLists>