PROCESS FOR PREPARING SEMISYNTHETIC ENZYMES

摘要

Disclosed is a process for chemically modifying a native protein to produce a biologically active modified enzyme-like protein. A solid support immobilized inhibitor of a model enzyme is prepared. A native protein is partially denatured and brought into contact with the support immobilized model enzyme inhibitor. A cross-linking agent is then added to the protein in the presence of the support immobilized model enzyme inhibitor and the nixture is incubated for a time and temperature sufficient to cross-link the native protein. The modified enzyme-like protein is removed from the cross-linking agent and the immobilized inhibitor and purified to produce a modified enzyme-like protein having the biological activity characteristics of the model enzymatic protein. The thusly prepared modified enzyme-like protein exhibits different biological activity than the starting native protein.