Process for producing protein, and vector, recombinant DNA and transformant used therefor.

摘要

Disclosed is a process for producing a protein, which comprises: (1) providing a gene which comprises a structural gene of a desired exogenous protein; (2) providing a vector which comprises a gene comprising a gene (A) coding for a signal peptide capable of linking the structural gene of the desired exogenous protein to said gene immediately after the end on the downstream side of the gene (A) and is proliferable in a predetermined host cell; (3) preparing a recombinant DNA proliferable in a predetermined host cell by use of the gene having the structural gene of the desired exogenous protein and said vector; (4) transforming the host cell by use of the recombinant DNA to prepare a transformant; and (5) culturing the transformant and recovering the desired exogenous protein produced. The exogenous or foreign protein produced within the host cell is excreted from the cell thanks to the signal peptide. The vector and the recombinant DNA for use in the process, and the resultant transformant are also claimed.