| 摘要 |
<p>In its broadest aspect, the present invention provides a method of effecting an electrochemical specific-binding assay of a ligand, either qualitatively or quantitatively, in an apparatus comprising at least one electrode, in which method a labelled component of the assay medium is, at least in part, magnetically held in the vicinity of the electrode. The electrochemical assay method may include a separation step, whereby bound label may be separated from free label in the assay medium. Preferably the electrochemical assay method will include the step of determining a perturbation in an electrochemical characteristic of components of the assay medium associated with a ligand complexing reaction.</p><p>Such an assay method is applicable to, for example, antibodies and antigens and suitable labels include redox centres, enzyme labels in the presence of an electron-transfer mediator and electron-transfer mediator Jabals in the presence of an electron donor or acceptor.</p> |
