| 摘要 |
<p>A column and detector are disclosed for two-dimensional column chromatography of a sample, in which the sample is injected close to a corner of a thin parallelepipedal column through which percolates a first solvent pumped at a controlled velocity. After a convenient time this flow is stopped and a stream of a second solvent is pumped at a controlled velocity in the perpendicular direction. Elution of the separated components is performed, the stream exiting from the column flows over a detector which monitors its composition at a large number of points, along the exit edge allowing the derivation of a two-dimensional chromatogram. The chromatographic bed can be honogeneous or made of a first portion including a first composition suitable to perform column chromatography in the first direction of the column and a second portion including a second composition suitable to perform column chromatography in a second direction of the column, wherein both compositions are selected from compositions available for column chromatography. A method for making a column and detector for three-dimensional chromatography is also disclosed. Methods for using such columns and detectors, over-pressure development of conventionally read two-dimensional thin column chromatograms, use of the thin-layer column as a multicolumn chromatograph, injection of unknowns into the flowing solvent, optimum flow rates, and a special cuvette plate and associated apparatus for performing the methods are also disclosed.</p> |
