发明名称 A method and device for aseptically propagating cells or tissues.
摘要 <p>The invention relates to a method for aseptically propagating cells and tissue, more particular for the vegetative propagation of plantlets, whereby in a sterile environment starting material is placed in individual membrane capsules, into which an amount of nutrient medium for the material to be propagated or to be rooted in advance, upon which the capsules are sealed. According to the invention a continuous capsule chain (2) is caused to gradually unwind from a flat folded storage condition (1) and to move in a substantially upright position along a number of stations, viz. a first station (A), where the capsule chain passes through the space between two upright walls (12), while an underpressure is created within the latter space, which, under pressure, causes the opposing membrane walls of the capsule chain (2) to spread apart, after which the thus opened capsule cells are filled with a quantity of nutrient medium with the aid of filling means which may be introduced from above; a second station (B), in which a plantlet to be propagated or rooted respectively is placed from above in each of the capsule cells which are previously provided with nutrient medium; a third station (24) in which the capsule cells containing culture material are closed by a horizontal seal and a fourth station, in which the filled and closed capsule chain is placed in a storage container in a horizontally packed condition.</p>
申请公布号 EP0412621(A1) 申请公布日期 1991.02.13
申请号 EP19900202160 申请日期 1990.08.08
申请人 PERMX B.V. 发明人 VAN OEVEREN, JACOBUS JOHANNES
分类号 A01H4/00;C12M3/00;C12N5/04 主分类号 A01H4/00
代理机构 代理人
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