| 摘要 |
PURPOSE:To prevent the deterioration of reactive reagents and to extend a shelf life by mixing the 1st reagent without contg. an alkali component and the 2nd reagent contg. the alkali component and bringing immediately the mixture thereof into reaction with a measuring sample and detecting the fluorescence thereof. CONSTITUTION:A sample such as amino acid is added to an eluate by a sampling valve 4 via a pump 3 from a vessel 1 and the liquid is fed to a chromatograph 5, by which the liquid is subjected to sepn. On the other hand, a fluorescent reagent (the 1st reagent) 7a without contg. the alkali (e.g., NaOH) and a fluorescent reagent (the 2nd reagent) 7b contg. the alkali are fed respectively as the reagents to generate the fluorescence by reacting with the sample by pumps 8a, 8b and are mixed with a valve 13. The separated sample 5 and the mixed fluorescence reagents 13 are mixed with each other in a valve 14 right after the mixing and are brought into reaction 9 to generate the fluorescence which is then analyzed 10. The fluorescence reagent without contg. the alkali and the fluorescence reagent contg. the alkali component are thus mixed right after the reaction with the sample and therefore the life of the fluorescence reagent is extended and the detection with high accuracy is made possible. |
