| 摘要 |
PURPOSE:The downstream part of the Escherichia coli triptophane promotor from the cleavage site by the restriction enzyme, Hpal, is replaced with a synthetic DNA fragment to give a DNA base sequence for high-efficiency development of exogenotes in Escerichia coli. CONSTITUTION:The downstream part of Escherichia coli triptophane promotor from the cleavage site by the restriction enzyme, Hpal, is replaced with a synthetic DNA fragment to the objective DNA base sequence that contains the Escherichia coli promotor, SD sequence, preferably the SD sequence of leader peptide gene of the Escherichia coli triptophane operon and the translation- starting codon where the TTT sequence is included right after the SD sequence or in partial duplication. The DNA base sequence is preferably TTTN<1>... ... ...N<n>ATG (N is deoxynucleotide; n is 3-12) downstream of the SD sequence. |
