IMMOBILIZED ENZYME FILM FOR ENZYME ELECTRODE

摘要

PURPOSE:To enable the reaction in accordance with the substrate concn. of a sample liquid even if said liquid contains dissolved oxygen at a low ratio by providing a hydrous layer on the surface of the immobilized enzyme layer of an immobilized enzyme film for an enzyme electrode on the side of the sample to be measured. CONSTITUTION:10wt% water soluble PVA dissolved in distilled water is coated on a protective film 1 consisting of, for example, a porous polycarbonate film and is dipped in a soln. mixture (1:1:1 mixing ratio) of 10wt% glutaraldehyde, 10wt% sodium sulfate and 1N hydrochloric acid and is thus brought into reaction therewith for 3hr at 40 deg.C temp. The hydrous layer 2 consisting of an unsatd. PVA layer having 3-6mum thickness is thus formed. The immobilized enzyme layer 3 and a permselective membrane 4 are successively formed on the layer 2 to constitute the immobilized enzyme film A. The measurement of the sample liquid contg. the dissolved oxygen at a low rate is thus made possible without saturation of the output and the practicality is improved.