| 摘要 |
A substrate consisting essentially of regenerated collagen fibrils is provided which is in the form of a bead, or microsphere and comprises irregularly entangled regenerated collagen fibrils each having a diameter of 10-1000 m mu and an aqueous solution existing between the regenerated collagen fibrils, the content of the regenerated collagen fibrils being 20-0.01 wt. %. The substrate can be used for cell culture or for measuring adhesion activity of blood platelet. According to one method of manufacturing the collagen beads, an acidic aqueous collagen solution is dispersed in a water-immiscible organic solvent in the form of numerous droplets to form an emulsion, and the droplets are then coagulated by addition of a water-miscible organic solvent and an alkali to the emulsion. According to another method of manufacturing the collagen beads or microspheres, a neutral collagen solution is dispersed in a water-immiscible organic solvent in the form of numerous droplets to form an emulsion, and the droplets are then coagulated by raising the temperature of the emulsion to 30 DEG C.-40 DEG C. The collagen beads or microspheres prepared by the methods described above may be cross-linked by hexamethylenediisocyanate or glutaraldehyde.
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