发明名称 |
CELL DIFFERENTIATION BASED UPON PARTICLE UPTAKE |
摘要 |
<p>CELL DIFFERENTIATION BASED UPON PARTICLE UPTAKE This invention relates to a method of enumerating and isolating mutant cells and other variant cells in a cell population. A cell population is treated with a selective agent which allows nonvariant cells to remain phagocytic while rendering non-variant cells non-viable or non-phaqocytic. A suspension of discriminator particles, such as fluorescent or magnetic particles, is mixed and incubated with the cells. The variant cells take up the particles, while the non-variant cells do not take up the particles. The cell population is then analyzed to determine which cells have taken up discriminator particles.</p> |
申请公布号 |
CA1199598(A) |
申请公布日期 |
1986.01.21 |
申请号 |
CA19830433881 |
申请日期 |
1983.08.04 |
申请人 |
MASSACHUSETTS INSTITUTE OF TECHNOLOGY |
发明人 |
KOMIVES, ELIZABETH A.;THILLY, WILLIAM G. |
分类号 |
G01N15/10;G01N15/14;(IPC1-7):C12Q1/04 |
主分类号 |
G01N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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