RECOMBINANT DNA, ITS PREPARATION, BACTERIAL STRAIN CONTAINING SAME, PREPARATION OF EXOCYTIC SECRETION ENZYME USING SAME, AND DNA FOR PROMOTING SECRETION OF EXOCYTIC ENZYME

摘要

PURPOSE:To obtain an exocytic secretion enzyme, having high yield based on the amount of the bacterial cell and containing little impurities, by recombining a chromosome DNA fragment of bacterial cell belonging to Bacillus genus to an ENA-introducing vector for Bacillus subtilis, and transforming the cell. CONSTITUTION:The chromosome DNA of a bacterial cell of Bacillus genus is fragmentized, and the resultant DNA fragment is combined in a DNA-introducing vector for Bacillus subtilis to obtain a recombinant DNA. A cell of Bacillus subtilis or its affinity is transformed with the recombinant DNA, and the cell having improved exocytic secretion of the exocytic secretion enzyme is selected from the transformed Bacillus subtilis or its affinity. Preferable example of the Bacillus genus strain used in the above process is Bacillus licheniformis. To improve the recombination efficiency of the recombinant DNA, it is preferable to incise the DNA-introducing vector with a restriction enzyme, and treating the vector with an alkali phosphatase.