| 摘要 |
PURPOSE:To obtain an instrument which detects a nucleic acid base with high accuracy by introducing a specific element which is not contained in natural nucleic acid into the nucleic acid (mixture) to be detected and analyzing the specific element in the nucleic acid fragment separated according to the mol.wt. with said nucleic acid as a sample. CONSTITUTION:The element which is not contained in natural nucleic acid or is contained extremely little therein, for example, a non-metallic element such as S, Br or I a metallic element such as silver, gold, platinum, Os or Hg is introduced into the nucleic acid to be detected without changing largely the chemical or biochemical properties of the nucleic acid. For example, the sample prepd. by substituting the O in the phosphate conjugated part of a DNA terminal with S is used. The sample soln. is put into a liquid chromatography column 14 contg. a packing material 14 from a sample soln. tank 1 and an eluate is passed from an eluate tank 15. The S in each sepn. zone is identified by providing a suitable analyzing means such as atomic absorption analysis, plasma emission analysis to a labeling element detecting part 9. The detection or quantitative determination of the nucleic acid base in a short period is thus made possible with the simple device. |
