EXTRUSION TECHNIQUES FOR PRODUCING LIPOSOMES

摘要

Extrusion techniques for producing populations of liposomes having a substantially unimodal size distribution and for producing unilamellar liposomes. The unimodal size distribution is achieved by repeatedly passing previously formed liposomes through one or a plurality of filters, all of which have the same pore size. The unilamellar liposomes are produced by using a filter which has a pore size equal to or less than about 100 nm. In accordance with other aspects of the disclosure, liposomes are prepared directly from a lipid powder or pellet and buffer without the use of any solvents, detergents or other extraneous materials.