发明名称 |
METHODS FOR AGROBACTERIUM-MEDIATED TRANSFORMATION |
摘要 |
The present invention relates to methods for improving the transformation frequency of Agrobacterium-mediated transformation of maize embryos. A preferred method for transforming maize using Agrobacterium comprises the steps of: contacting at least one immature embryo from a maize plant with Agrobacterium capable of transferring at least one gene to said embryo; cocultivating the embryos with Agrobacterium; culturing the embryos in medium comprising N6 salts, an antibiotic capable of inhibiting the growth of Agrobacterium, and a selective agent to select for embryos expressing the gene; and regenerating plants expressing the gene.
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申请公布号 |
CA2278618(A1) |
申请公布日期 |
1998.07.30 |
申请号 |
CA19982278618 |
申请日期 |
1998.01.23 |
申请人 |
PIONEER HI-BRED INTERNATIONAL, INC. |
发明人 |
ZHAO, ZUO-YU;CAI, TISHU;PIERCE, DOROTHY A.;GU, WEINING |
分类号 |
C12N15/82;C12N15/84;(IPC1-7):A01H4/00;A01H5/00 |
主分类号 |
C12N15/82 |
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