| 摘要 |
The present invention is directed to a process for the production of a modified phytase with improved activity properties characterized therein that the following steps are effected: a) the three dimensional structure of the phytase to be modified and, optionally of another phytase with activity properties which are more favorable than the ones of the phytase to be modified is/are computer modelled on the basis of the three dimensional structure of the phytase of Aspergillus niger; b) the structure of the active sites of the phytase to be modified and of the phytase with the more favorable activity properties are compared and those amino acid residues in both active sites which are different are identified; c) a DNA sequence coding for a modified phytase is constructed by changing the nucleotides coding for at least one of the amino acids by which both active sides differ; d) integrating such a DNA sequence into a vector capable of expression in a suitable host cell; e) transforming a suitable host cell by the DNA sequence of c) or the vector of d), growing said host cell under suitable growth conditions and isolating the modified phytase from the host cell or the culture medium by methods known in the state of the art. The present invention is also directed to phytases obtainable or obtained by such a process. |
