| 摘要 |
PURPOSE:To obtain 5 kinds of separated soyasaponins, in high purity, by extracting processed soybean, etc., removing impurities from the extract by a specific means, and purifying the resultant mixed soyasaponin by high performance liquid chromatography. CONSTITUTION:Powder of soybean material such as (semi)processed soybean, waste of soybean processing step, etc. is added with methanol, and refluxed under heating for several hours. The solution is centrifuged, the obtained extract liquid is partitioned with n-butanol and water, and the extract liquid of the n- butanol layer is adsorbed by column chromatography. The adsorbed components are eluted successively by changing the organic solvents to remove the impurities. The obtained solution containing soyasaponins is concentrated in vacuum. The mixture of 5 kinds of soyasaponins obtained by the above process is purified at a high speed by high-performance liquid chromatography, and 5 kinds of soyasaponins are separated from each other by the difference of the retention time. |
