| 摘要 |
PURPOSE:To express efficiently the aimed gene, by linking the aimed gene to the downstream side of a promoter obtained from a long terminal repeat (LTR) part derived from a baboon endogenous virus, and introducing the resultant vector into human cells. CONSTITUTION:A plasmid pBM3.2 obtained by cloning a long terminal repeat (LTR) derived from a baboon endogenous virus in a BamHI site of an Escherichia coli plasmid pBR322 is cleaved with Bgl II, partially decomposed to give a plasmid pBM-BI deficient in BamHI(A) to Bgl II(B), which is then cleaved with EcoRI and Sal I to carry out agarose electrophoresis to afford a DNA fragment containing the LTR as the aimed promoter. Human cells are transformed with a vector having a gene, e.g. interleukin 2, linked to the downstream side of the promoter and cultivated. |
