| 摘要 |
PURPOSE:To realize a highly reproducible assay with a simple operation, by making N-(2,4-dinitrophenyl) cysteine react with a substance containing a thiol group reacting group. CONSTITUTION:N-(2,4-dinitrophenyl) cysteine is made to react with a substance containing a thiol group reacting group and the absorbance derived from N-2,4- dinitrophenyl group in the resultant product is measured to assay the thiol group reacting group. The thiol group reacting group herein used is a maleimide group, 2,5-dioxyso-3-cyclohexenyl group, iodoacetyl group, bromoacetyl group or chloroacetyl group and the substance containing the thiol group reacting group is water-soluble protein introducing the thiol group reacting group. This method eliminates troublesome procedure for preventing errors due to disulfide conversion as in the case of using a thiol compound thereby making it sufficiently applicable for industrial production basis. |
