ENERGY ABSORBING PARTICLE QUENCHING IN LIGHT EMITTING COMPETITIVE PROTEIN BINDING ASSAYS

摘要

Assays for low concentrations of a member of a specific binding pair are provided employing fluorescent particles and absorbent particles, wherein the absorbent particles substantially inhibit fluorescence only when bound to the fluorescent particles through specific non-covalent binding. Thea amount of binding is a function of the amount of a specified analyte in the aqueous medium in which the assay takes place; the amount of analyte in the medium can therefore be quantified by comparison with medium having a standard amount of analyte. The assays is applicable to a wide range of materials having physiological activity, including natural and synthetic drugs and disease-related materials such as cells and viruses.