发明名称 |
Hybrid viral plasmid and microorganisms containing same |
摘要 |
pML BK is a hybrid plasmid which is constructed of :pML which contains the entire nucleotide sequence of the plasmid pBR322 except for deletion of nucleotide sequences 1120-2490 bp and 0 to 375 bp, and BK which contains the entire nucleotide sequence of the Gardner strain of BK virus except for deletion of nucleotide sequences between bp 5089-5196. Into the pML BK plasmid may be inserted a viral or human gene X to be expressed, an example of which is TK which represents the Bam HI generated 3,600 bp DNA fragment of herpes simplex virus type 1 which contains the herpes simplex virus type 1 TK DNA. The majority of the pML BK X DNA molecules remain in the episomal or free state in the human cells 143B and express the gene X.
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申请公布号 |
US4562155(A) |
申请公布日期 |
1985.12.31 |
申请号 |
US19830496000 |
申请日期 |
1983.05.19 |
申请人 |
THE WISTAR INSTITUTE OF ANATOMY AND BIOLOGY |
发明人 |
RICCIARDI, ROBERT P.;BARBANTI-BRODANO, GIUSEPPE;MILANESI, GABRIELE |
分类号 |
C12N15/86;(IPC1-7):C12N15/00;C12N5/00;C12N1/20;C12N1/00 |
主分类号 |
C12N15/86 |
代理机构 |
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