OPTIMAL POLYPEPTIDE PRODUCTION

摘要

the Disclosure Compositions and methods featuring, in one aspect, a method of providing a fused gene having a promoter located an optimal distance from the translation start site, the method including providing a fused gene having a region of a gene coding for a eukaryotic or prokaryotic polypeptide fused to a region of a gene coding for an assayable polypeptide, inserting a portable promoter at varying distances in front of the translation start site, cloning the fused genes into microorganisms, selecting those producing the greatest amount of assayable polypeptide, and reconstituting the gene for the eukaryotic or prokaryotic protein.