| 摘要 |
PURPOSE:To enable convenient and accurate assay of specified deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) by using labelled polynucleotide. CONSTITUTION:Single-stranded polynucleotide to be in contact with a specimen polynucleotide has a marker-containing high-molecular compound bonded to the 5'- or 3'-terminal thereof and combines with a specimen single-stranded polynucleotide to form a double-stranded polynucleotide. The above single stranded polynucleotide can be synthesized by the solid phase polynucleotide synthesis method or gamma-DNA method according to genetic engineering technology using plasmid. The above-mentioned high-molecular compound may be any one which is water-soluble and does not enter into non-specific reaction with the specimen polynucleotide, pref. one with MW of 1000 or more. The labelled polynucleotide is brought into contact with the specimen polynucleotide to form a double- stranded polynucleotide. After the reaction, the marker adsorbed on a filter is assayed by conventional analytical methods corresponding to the kind thereof. |
