| 摘要 |
FIELD: medicine, immunology. ^ SUBSTANCE: invention relates to methods for in vitro diagnosis of infection caused by microorganism, and using reagents and sets for detection of antigens and antibodies raised against infectious microorganism. Method involves carrying out simultaneous detection of microorganism antigen and antibodies raised against this antigen. For detection of infection method involves carrying out contacting biological sample with a binding antibody raised against microorganism and a binding antigen from this microorganism. Mixture is incubated and formed complexes "antigen-antibody" are revealed using labeled and, if necessary, detecting antibody and/or detecting antigen. Binding and/or labeled detecting antigen comprises fragment of microorganism antigen wherein at least one epitope of that is inactivated, and a binding and/or detecting antibody recognizes native epitope of bound antigen. Also, invention proposes a set for detection of infection and peptides or polypeptides of hepatitis C and HIV proteins using of that allows carrying out a method for diagnosis of these diseases. Using the proposed method allows enhancing precision of diagnosis of infection caused by microorganism. ^ EFFECT: improved detecting method. ^ 42 cl, 13 tbl, 2 dwg, 8 ex |
