| 摘要 |
FIELD: analytical chemistry, biology, toxicology, veterinary science. ^ SUBSTANCE: invention relates to a method for determination of N-(benzimidazolyl-2)-O-methylcarbamate in biological material. Method involves milling biological material sample, three-fold infusion with a mixture of solvent ethyl acetate-dichloroethane-formic acid taken in the ratio 5:5:1 (by volume), respectively, as an organic extractant, each time for 15 min. Prepared extracts are separated from solid particle of biological material, combined, combined extract is filtered and filtrate is extracted with 0.1 N hydrochloric acid solution. Prepared acid extract is washed out with ethyl acetate as hydrophobic organic solvent, ethyl acetate fraction is discarded, aqueous fraction is alkalinized with 10% sodium hydroxide solution to pH value 8-9 and extracted with ethyl acetate. Prepared extract is separated, dehydrated, extractant is evaporated and residue is dissolved in organic solvent (glacial acetic acid). Obtained residue is subjected for chromatography in silica gel thin layer treated with vaseline oil using a mixture acetonitrile-water (6:4) as a mobile phase. Chromatograms are developed in UV-light, analyzed substance is eluted from sorbent with a mixture solvents ethyl acetate-dichloroethane-formic acid (5:5:1) and optical density of eluate is determined at wavelength 282 nm. Method provides enhancing extraction degree, precision and sensitivity of assay. Invention can be used in practice of sanitation-epidemiology departments, chemical-toxicology and veterinary laboratories. ^ EFFECT: improved method of determination. ^ 3 tbl, 2 ex |
