STRAIN OF HYBRID CULTURED MAMMALIAN CELLS MUS MUSCULUS AS PRODUCER OF MONOCLONAL ANTIBODIES TO CONFORMATION-DEPENDENT DETERMINANTS OF TUMOR-ASSOCIATED HUMAN ANTIGEN MUC I

摘要

FIELD: biotechnology, immunology, medicine, oncology. ^ SUBSTANCE: strain of hybrid cultured mammalian cells Mus musculus VKPM H-97 is prepared by the hybridoma technology method. This strain represents a producer of monoclonal antibodies possessing specificity to conformation-dependent of tumor-associated human antigen Muc I. Productivity of the strain and specificity of produced antibodies is estimated based on immunoenzyme assay using some markers of specificity: natural purified antigen Muc I isolated from human milk; VNTR22-polypeptide; synthetic monomeric polypeptide (TR1); deglycosidated antigen Muc I (de-Muc I) prepared by chemical oxidation of natural Muc I; hypoglycosidated antigen Muc I (o-Muc I) prepared by periodate oxidation of natural Muc I. Monoclonal antibodies produced by the claimed strain recognize clinically significant isoforms of Muc I antigen and allows assaying its concentration in human serum blood in carrying out early diagnosis. Invention can be used for preparing monoclonal antibodies to tumor-associated human antigen Muc I. ^ EFFECT: valuable properties of strain. ^ 2 dwg, 3 ex