| 摘要 |
PURPOSE:To obtain a stable tissue plasminogen activator (6-PA for short) useful as a thrombolytic drug, showing no reduction in activity even after long-period preservation, by adding purified gelatin to a single stranded t-PA. CONSTITUTION:Purified gelatin is added to a single stranded t-PA, so that the single stranded t-PA is stabilized. An acid-treated gelatin having 7-9 isoelectric point is preferable as the gelatin and, a low-molecular gelatin having 4,000- 20,000 average molecular weight is used to avoid antigenicity. An amount of the gelatin added is preferably 0.5-5W/V% based on pharmaceutical preparation. A single stranded t-PA of natural type existing in an organism is preferable as the t-PA, but conversion of it to duplex t-PA is advanced during purification process. Now, it is found that by adding purified gelatin, the single stranded t-PA causes hardly reduction in activity, and conversion of it to the duplex t-PA can be prevented even during long-period storage.
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