| 摘要 |
Factor VIII is purified by adsorption onto a insoluble matrix having free sulphate groups, such as dextran sulphate, and selective elution therefrom. A suitable eluant for the purification of the von Willebrand protein (Factor VIIIR:vWp) is citrate buffer, pH 6.85, containing 0.47 M sodium chloride and 2.14 mM calcium chloride. A suitable eluant for the purification of the Factor VIII complex (Factor VIIIR:Ag, Factor VIIIR:vWp and Factor VIII:C) is citrate buffer at pH value between 6.2 and 7.3 containing 1.0 M glycine, 2.14 mM calcium chloride and 0.5 M sodium chloride at +4 DEG C. |
