| 摘要 |
PURPOSE:To remove efficiently a pyrogen and obtain immunoglobulin effectively utilizable as a preventing and treating agent for various infectuous diseases, by adding polyethylene glycol to immunoglobulin containing the pyrogen under specific conditions. CONSTITUTION:Immunoglobulin containing a pyrogen, e.g. produced by microorganisms such as bacteria, yeast or molds, is dissolved to give a solution at 6-8pH. The pH of the immunoglobulin is usually within the above-mentioned range, and adjustment is not particularly required. However, the pH can be adjusted with sodium hydroxide, hydrochloric acid, etc. if necessary. Polyethylene glycol is then added to the resultant solution and stirred to give a uniform solution, which is allowed to stand preferably at 15-25 deg.C, perferably about 19-21 deg.C for about 30-60min to precipitate the pyrogen. The precipitated pyrogen is separated by centrifugation, filtration, etc. The amount of the polyethylene glycol to be added is preferably about 4.0-5.5% in the case of 4,000 molecular weight.
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