Site-specific proteolysis by renin.

摘要

A nucleotide sequence which codes for an amino acid sequence specifically recognized and cleaved by the endopeptidase, renin, has been inserted into a plasmid expression vector. This nucleotide sequence was placed within a sequence of DNA, at the junction between the sequences coding for two distinct domains of a fusion protein. The resultant novel protein product expressed by the new construction can be specifically cleaved by renin to yield two distinct proteins corresponding to the two domains of the original fusion protein product.