发明名称 |
QUALITATIVE AND/OR QUANTITATIVE DETERMINATION OF A PROTEINACEOUS MOLECULE IN A PLURALITY OF SAMPLES |
摘要 |
<p>The present invention relates to the qualitative and/or quantitative determination of a proteinaceous molecule in a plurality of samples by combining a particular labeling and fractionation strategy. In particular, each sample is provided with a dye chosen from a set of dyes, wherein each dye within the set of dyes emits luminescent light at a wavelength that is sufficiently different from the emitted luminescent light of the remaining dyes in the set of dyes to provide a different light signal when excited. Subsequent to labelling the proteinaceous molecule with the fluorescent dye, the samples are combined and fractionated by means of electrophoresis, the labeled proteinaceous molecule having a relative electrophoretic mobility that differs from the electrophoretic mobility of the proteinaceous molecule labeled with another dye within the set of dyes. After capturing separate images of the labeled proteinaceous molecule at different wavelengths, the images are aligned by means of image processing. The present invention further relates to a kit and a set of luminescent dyes for use in qualitatively and/or quantitatively determining a proteinaceous molecule in a plurality of samples by means of electrophoresis.</p> |
申请公布号 |
WO2010010158(A1) |
申请公布日期 |
2010.01.28 |
申请号 |
WO2009EP59513 |
申请日期 |
2009.07.23 |
申请人 |
LEIBNIZ-INSTITUT FUER PFLANZENBIOCHEMIE (IPB);NAUMANN, KAI;SCHEEL, DIERK |
发明人 |
NAUMANN, KAI;SCHEEL, DIERK |
分类号 |
G01N27/447 |
主分类号 |
G01N27/447 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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