UN PROCEDIMIENTO PARA DETECTAR ANTIGENOS DE ESTREPTOCOCO BETA-HEMOLITICO EN UN ESPECIMEN CLINICO RECOGIDO EN UNA TORUNDA, SIN CENTRIFUGACION

摘要

A method for detecting beta-hemolytic streptococcus antigens in clinical specimens wherein the antigen is extracted from an uncentrifuged clinical specimen by means of nitrous acid, the extracted specimen is adjusted to an approximately neutral pH and is brought into contact with an appropriate latex agglutination reagent. In preferred practice the specimen is collected on a throat swab and the entire extraction and neutralization steps are effected in a microtube in the presence of the swab. The swab is then employed to remove the neutralized, extracted specimen to a slide or the like onto which a few drops of the specimen are expressed by rolling the swab. Detection of the antigen by conventional latex agglutination techniques may then be accomplished optimally using latex spheres onto which high titer rabbit gamma globulin is passively adsorbed.