| 摘要 |
PURPOSE:Human leukemia cell is cultured in the presence of a differentiation- inducing agent, and the titled substance is separated from the resultant culture liquid. CONSTITUTION:Human leukemia cell (e.g. human-originated monocytic leukemia cell J-111, acute human promyelocytic leukemia cell HL-60, acute human lymphoblastic leukemia cell CCRF-SB, etc.) is cultured in the presence of a differentiation-inducing agent (e.g. 12-O-tetradecanoylphorbol-13-acetate, etc.). In the above process, it is preferable to proliferate the human leukemia cell preliminary by the preculture in a culture medium, and carry out the main culture by adding the differentiation-inducing agent to the medium containing the proliferated cells. The preculture and the main culture are carried out aerobically at 30-40 deg.C, and an agent to promote and stimulate the secretion (e.g. zymosan, yeast cell component, etc.) is added to the culture liquid. The objective substance active to damage cancer cell can be separated from the culture liquid e.g. by centrifugal separation. The substance is a protein having extremely high safety and stable to the heat-treatment at 56 deg.C for 30min.
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