发明名称 |
Interleukin-2 receptor and DNA encoded therefor and their preparation. |
摘要 |
<p>Interleukin-2 receptor derived from normal and malignant cells has been purified by use of various techniques including affinity chromatography in conjunction with a monoclonal antibody directed to the receptor. The purification process also includes reversed phased high performance liquid chromatography. By these techniques, interleukin-2 receptor has been purified to homogeneity. The high purification of the interleukin-2 receptor has made possible the sequencing of the amino acid residues at the N-terminal of this protein molecule. Double-stranded cDNA is prepared from polyadenylated RNA extracted from cell lines or other sources known to produce IL-2 receptor. The cDNA is inserted within a plasmid vector and then the recombinant plasmid employed to transform an appropriate host. Transformed hosts are identified and grouped into pools. Plasmid DNA prepared from these pools is hybridized with a labeled synthetic oligonucleotide probe corresponding to a portion of the amino acid sequence of the purified IL-2 receptor. The cDNA clone isolated with the probe is characterized by restriction enzyme mapping and sequenced by chain-termination method. The particular DNA clone that actually contains the gene coding for the functional IL-2 receptor is identified by expressing the clones in COS-7 monkey kidney cells and assaying for the expressed IL-2 receptor by its ability to bind IL-2 or a monoclonal antibody directed against the IL-2 receptor.</p> |
申请公布号 |
EP0162699(A2) |
申请公布日期 |
1985.11.27 |
申请号 |
EP19850303575 |
申请日期 |
1985.05.21 |
申请人 |
IMMUNEX CORPORATION |
发明人 |
CERRETTI, DOUGLAS P.;COSMAN, DAVID J.;DOWER, STEVEN K.;MARCH, CARL J.;URDAL, DAVID L.;LARSEN, ALF D. |
分类号 |
A61K38/00;C07K14/00;C07K14/705;C07K14/715;C07K16/28;C12N15/09;C12P21/02;C12R1/91;(IPC1-7):C12N15/00;C07K13/00 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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