SYNTHETIC SUBSTRATE AND ASSAY METHOD OF ENZYME ACTIVITY

摘要

Provided by this invention are amide compounds of the formula < IMG > (I) where R1 is a lower alkyl group and R2 is a substituted methylamino group or a D-amino acid residue, and salts thereof. These compounds are useful in the assaying of leucine aminopeptidase (LAP). The invention also provides an assay method for leucine aminopeptidase which comprises reacting a substrate amide compound or salt thereof of the formula (I) < IMG > wherein R1 is a lower alkyl group and R2 is a substituted methylamino group or a D-amino acid residue, with a sample for leucine aminopeptidase assay, incubating the reaction product substituted methylamine compound or D-amino acid with the corresponding oxidase, and measuring the thus consumed oxygen or liberated hydrogen peroxide.